CLTB Monoklonaler Antikörper

CLTB Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F9C2

Kat-Nr. : 66270-1-PBS

Synonyme

Clathrin light chain B, clathrin, light chain (Lcb), CLTB, LCB

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  WB analysis of pig brain using 66270-1-Ig (same clone as 66270-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66270-1-Ig (CLTB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66270-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66270-1-Ig (same clone as 66270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66270-1-Ig (CLTB Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66270-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66270-1-Ig (same clone as 66270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66270-1-Ig (CLTB Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66270-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66270-1-Ig (same clone as 66270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66270-1-Ig (CLTB Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66270-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66270-1-Ig (same clone as 66270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66270-1-Ig (CLTB Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66270-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66270-1-Ig (same clone as 66270-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66270-1-Ig(CLTB antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66270-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66270-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CLTB und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CLTB fusion protein Ag24347
• Vollständiger Name: clathrin, light chain (Lcb)
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 32-38 kDa
• GenBank-Zugangsnummer: BC006457
• Gene symbol: CLTB
• Gene ID (NCBI): 1212
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)