CLTC Monoklonaler Antikörper

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CLTC Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1B3D7

Kat-Nr. : 66487-1-PBS

Synonyme

1B3D7, CHC, CHC17, Clathrin heavy chain, Clathrin heavy chain 1

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  WB analysis of HeLa using 66487-1-Ig (same clone as 66487-1-PBS)

  WB result of CLTC antibody (66487-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLTC transfected HeLa cells. This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

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  WB analysis using 66487-1-Ig (same clone as 66487-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66487-1-Ig (CLTC antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

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  WB analysis using 66487-1-Ig (same clone as 66487-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66487-1-Ig (CLTC antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66487-1-Ig (same clone as 66487-1-PBS)

  HeLa, Jurkat, ROS1728 cells, and pig brain tissue were subjected to SDS PAGE followed by western blot with 66487-1-Ig (CLTC antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 66487-1-Ig (same clone as 66487-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66487-1-Ig (CLTC antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 66487-1-Ig (same clone as 66487-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 66487-1-Ig (same clone as 66487-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 66487-1-Ig (same clone as 66487-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 66487-1-Ig (same clone as 66487-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66487-1-Ig (same clone as 66487-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66487-1-Ig (same clone as 66487-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 66487-1-Ig (same clone as 66487-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 66487-1-Ig (same clone as 66487-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66487-1-Ig (same clone as 66487-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CLTC antibody (66487-1-Ig, Clone: 1B3D7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66487-1-Ig (same clone as 66487-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66487-1-Ig (CLTC antibody), at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66487-1-PBS bindet in WB, IHC, IF/ICC, ELISA CLTC und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CLTC fusion protein Ag25040
• Vollständiger Name: clathrin, heavy chain (Hc)
• Berechnetes Molekulargewicht: 192 kDa
• Beobachtetes Molekulargewicht: 170 kDa
• GenBank-Zugangsnummer: BC054489
• Gene symbol: CLTC
• Gene ID (NCBI): 1213
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (CLTC) (160-190 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The heavy chain provides the structural backbone of the clathrin lattice. (PMID: 9133677)