CLTCL1 Monoklonaler Antikörper

CLTCL1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B5A10

Kat-Nr. : 66274-1-PBS

Synonyme

CHC22, Clathrin heavy chain 2, clathrin, heavy chain like 1, CLH 22, CLH22, CLTCL, CLTCL1, CLTD

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  WB analysis of HeLa using 66274-1-Ig (same clone as 66274-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66274-1-Ig (CLTCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66274-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66274-1-Ig (same clone as 66274-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66274-1-Ig (CLTCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66274-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66274-1-Ig (same clone as 66274-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66274-1-Ig (CLTCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66274-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 66274-1-Ig (same clone as 66274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66274-1-Ig (CLTCL1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66274-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 66274-1-Ig (same clone as 66274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66274-1-Ig (CLTCL1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66274-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66274-1-Ig (same clone as 66274-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 66274-1-Ig (CLTCL1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66274-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66274-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CLTCL1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: clathrin, heavy chain-like 1
• Berechnetes Molekulargewicht: 187 kDa
• Beobachtetes Molekulargewicht: 180 kDa
• GenBank-Zugangsnummer: NM_007098
• Gene symbol: CLTCL1
• Gene ID (NCBI): 8218
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CLTCL1, also named as CLH22, CLTCL and CLTD, belongs to the clathrin heavy chain family. Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. The antibody is specific to CLTCL1. It has no cross reaction to CLTC.