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Clusterin Monoklonaler Antikörper

Clusterin Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

CloneNo.

2F12G10

Kat-Nr. : 66109-1-Ig

Synonyme

AAG4, Apo J, APOJ, ApoJalpha, ApoJbeta, Apolipoprotein J, CLI, CLU, clusterin, Complement cytolysis inhibitor, Ku70 binding protein 1, KUB1, NA1/NA2, SGP 2, SGP2, SP 40, TRPM 2, TRPM2

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of human plasma using Clusterin Monoclonal antibody (66109-1-Ig)

    WB analysis of human plasma using 66109-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66109-1-Ig (Clusterin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using Clusterin Monoclonal antibody (66109-1-Ig)

    IHC staining of human liver using 66109-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Clusterin Monoclonal antibody (66109-1-Ig)

    IHC staining of human liver using 66109-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Clusterin Monoclonal antibody (66109-1-Ig)

    IHC staining of human breast cancer using 66109-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Clusterin Monoclonal antibody (66109-1-Ig)

    IHC staining of human breast cancer using 66109-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Clusterin Monoclonal antibody (66109-1-Ig)

    IHC staining of human prostate cancer using 66109-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Clusterin Monoclonal antibody (66109-1-Ig)

    IHC staining of human prostate cancer using 66109-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 40x lens).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBhuman plasma
Erfolgreiche Detektion in IHChumanes Lebergewebe, humanes Mammakarzinomgewebe, humanes Prostatakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66109-1-Ig bindet in WB, IHC,ELISA Clusterin und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Clusterin fusion protein Ag19236
Vollständiger Name clusterin
Berechnetes Molekulargewicht 449 aa, 53 kDa
Beobachtetes Molekulargewicht 36 kDa
GenBank-ZugangsnummerBC010514
Gene symbol CLU
Gene ID (NCBI) 1191
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). It is encoded by one single gene and cleaved posttranslationally into α (34-36 kDa) and β (36-39 kDa) subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement (PMID: 10694874).

Protokolle

Produktspezifische Protokolle
WB protocol for Clusterin antibody 66109-1-IgProtokoll herunterladen
IHC protocol for Clusterin antibody 66109-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen