CLUAP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CLUAP1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 17470-1-AP

Synonyme

Qilin, KIAA0643, Clusterin-associated protein 1, clusterin associated protein 1

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  WB analysis of mouse testis using 17470-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17470-1-AP (CLUAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17470-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17470-1-AP (CLUAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 17470-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 17470-1-AP (CLUAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17470-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17470-1-AP (CLUAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 17470-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17470-1-AP (CLUAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 17470-1-AP

  IP result of anti-CLUAP1 (IP:17470-1-AP, 4ug; Detection:17470-1-AP 1:600) with HepG2 cells lysate 2400ug.

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  IF Staining of MDCK using 17470-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CLUAP1 antibody (17470-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, COLO 320-Zellen, HepG2-Zellen, Y79-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IF/ICC: MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Produktinformation

17470-1-AP bindet in WB, IF/ICC, IP, ELISA CLUAP1 und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CLUAP1 fusion protein Ag11419
• Vollständiger Name: clusterin associated protein 1
• Berechnetes Molekulargewicht: 413 aa, 48 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC017070
• Gene symbol: CLUAP1
• Gene ID (NCBI): 23059
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CLUAP1 (Clusterin-associated protein 1) was originally identified as a protein that interacts with CLU/clusterin, and whose gene is frequently upregulated in colon cancer (PMID: 15480429). Overexpression of CLUAP1 was also observed in osteosarcoma (PMID: 17203229). CLUAP1 is most abundantly expressed in testis among normal tissues (PMID: 15480429; 17203229). Studies reveal that CLUAP1 has a role in ciliogenenesis and cilia-dependent development (PMID: 23351563; 23742838; 24970261).

Publikationen

Species	Application	Title
human	WB	Dev Cell; The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.; Authors - Tomoharu Kanie
human	WB,IF	Mol Cell Proteomics; CRISPR/Cas9-mediated genomic editing of Cluap1/IFT38 reveals a new role in actin arrangement.; Authors - Tina Beyer ; KO Validated
human	WB	Hum Mol Genet; Defective IFT57 underlies a novel cause of Bardet-Biedl syndrome; Authors - Alexandra Nitoiu
human	IF	Elife; A hierarchical pathway for assembly of the distal appendages that organize primary cilia; Authors - Tomoharu Kanie