CLUH Rekombinanter Antikörper

CLUH Rekombinant Antikörper für IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241640C11

Kat-Nr. : 84321-4-RR

Synonyme

KIAA0664, Protein KIAA0664, FLJ23672, CLU1, 241640C11

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  IF Staining of HeLa using 84321-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CLUH antibody (84321-4-RR, Clone: 241640C11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84321-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84321-4-RR against Human CLUH were performed. The affinity constant is 7.19 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84321-4-RR bindet in IF/ICC, ELISA CLUH und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CLUH fusion protein Ag34275
• Vollständiger Name: KIAA0664
• GenBank-Zugangsnummer: BC156940
• Gene symbol: CLUH
• Gene ID (NCBI): 23277
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Clustered mitochondria homolog (CLUH) is a conserved RNA-binding protein, that binds transcripts encoding proteins involved in the respiratory chain, the tricarboxylic acid (TCA) cycle, and other mitochondrial metabolic pathways. Upon loss of CLUH, target mRNAs are subjected to faster decay, and their respective proteins are decreased in abundance. This leads to alterations in mitochondrial distribution (mitochondrial clustering as the gene name alludes to) mitochondrial cristae integrity, respiratory defects, loss of mtDNA, and decreased activity of TCA cycle enzymes. (PMID: 35559794)