CLYBL Rekombinanter Antikörper

CLYBL Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242785F10

Kat-Nr. : 85399-4-RR

Synonyme

(3S)-malyl-CoA thioesterase, Beta-methylmalate synthase, Citramalyl-CoA lyase, mitochondrial, citrate lyase beta like, Citrate lyase beta-like

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  WB analysis using 85399-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85399-4-RR (CLYBL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 85399-4-RR

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 85399-4-RR (CLYBL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 85399-4-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 85399-4-RR (CLYBL antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85399-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85399-4-RR against Human CLYBL were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85399-4-RR bindet in WB, IHC, ELISA CLYBL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CLYBL fusion protein Ag11283
• Vollständiger Name: citrate lyase beta like
• Berechnetes Molekulargewicht: 340 aa, 37 kDa
• Beobachtetes Molekulargewicht: 30-40 kDa
• GenBank-Zugangsnummer: BC034360
• Gene symbol: CLYBL
• Gene ID (NCBI): 171425
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CLYBL, also named CLB, is a Mitochondrial citramalyl-CoA lyase indirectly involved in vitamin B12 metabolism (PubMed:29056341). It is a human mitochondrial enzyme of unknown function that is found in multiple eukaryotic taxa and conserved to bacteria. It acts as a beta-methylmalate synthase in vitro, by mediating the conversion of glyoxylate and propionyl-CoA to beta-methylmalate. There are two isoforms with MW 31-33 kDa and 35-37 kDa.