CMAS Polyklonaler Antikörper

CMAS Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13006-1-PBS

Synonyme

CMP Neu5Ac SYNTHETASE, CMP NeuNAc synthase, CMP-N-acetylneuraminic acid synthase, CMP-NeuNAc synthase, EC:2.7.7.43

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  WB analysis using 13006-1-AP (same clone as 13006-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13006-1-AP (CMAS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13006-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13006-1-AP (same clone as 13006-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13006-1-AP (CMAS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13006-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 13006-1-AP (same clone as 13006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13006-1-AP (CMAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13006-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 13006-1-AP (same clone as 13006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13006-1-AP (CMAS antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13006-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 13006-1-AP (same clone as 13006-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CMAS antibody (13006-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 13006-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13006-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CMAS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CMAS fusion protein Ag3686
• Vollständiger Name: cytidine monophosphate N-acetylneuraminic acid synthetase
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC016609
• Gene symbol: CMAS
• Gene ID (NCBI): 55907
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytidine monophosphate N-acetylneuraminic acid synthetase (CMAS), a key enzyme in the incorporation pathway, catalyzes the synthesis of CMP-sialic acid by utilizing CTP and sialic acid. Neu5Ac is activated in the nucleus by CMAS to form CMP-Neu5Ac, which is transported to the Golgi apparatus by SLC35A1 for sialylation of glycoproteins and gangliosides catalyzed by specific sialy ltransferases (PMID: 30568043). Low CMAS gene expression correlated with reduced recurrence-free survival in a human colorectal cancer cohort (PMID: 30578646). Western blots indicated a range of bands at ~ 48 kDa.