CMIP Polyklonaler Antikörper

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• KD/KO Validated

CMIP Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 12851-1-AP

Synonyme

c Maf inducing protein, c Mip, CMIP, KIAA1694, Tc Mip, TCMIP

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 12851-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12851-1-AP (CMIP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 12851-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12851-1-AP (CMIP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 12851-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12851-1-AP (CMIP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12851-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12851-1-AP (CMIP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 12851-1-AP

  IP Result of anti-CMIP (IP:12851-1-AP, 4ug; Detection:12851-1-AP 1:300) with Raji cells lysate 2000ug.

• 

  IHC staining of human gliomas using 12851-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12851-1-AP (CMIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12851-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12851-1-AP (CMIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12851-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12851-1-AP (CMIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12851-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12851-1-AP (CMIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12851-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12851-1-AP (CMIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12851-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12851-1-AP (CMIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12851-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12851-1-AP (CMIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12851-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12851-1-AP (CMIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 12851-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12851-1-AP (CMIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 12851-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12851-1-AP (CMIP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Raji-Zellen
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Lungenkarzinomgewebe, humanes Lymphomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

12851-1-AP bindet in WB, IP, IHC, IF, ELISA CMIP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CMIP fusion protein Ag3475
• Vollständiger Name: c-Maf-inducing protein
• Berechnetes Molekulargewicht: 86 kDa
• Beobachtetes Molekulargewicht: 86 kDa
• GenBank-Zugangsnummer: BC038113
• Gene symbol: CMIP
• Gene ID (NCBI): 80790
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The gene of CMIP was isolated from one patient of minimal change nephrotic syndrome. The gene encodes two isoforms: CMIP and truncated CMIP. The predicted CMIP and truncated CMIP proteins contain 739 and 679 amino acids and have calculated molecular masses of 85 and 75 kD, respectively. The two isoforms have different distribution and expression pattern.

Publikationen

Species	Application	Title
mouse	WB,IF	Cell Signal; Inhibition of nephrin activation by c-mip through Csk-Cbp-Fyn axis plays a critical role in Angiotensin II-induced podocyte damage.; Authors - Yu Lixia L ; KD Validated
mouse	WB	Cell Physiol Biochem; Induction of C-Mip by IL-17 Plays an Important Role in Adriamycin-Induced Podocyte Damage.; Authors - Yanbo Liu
human	WB,IHC	Biomed Res Int; CMIP Promotes Proliferation and Metastasis in Human Glioma.; Authors - Bin Wang ; KD Validated
human	WB,IHC	Pathol Res Pract; PMID: 31822364; Authors - Ru Xiang ; KD Validated
		Mol Med Rep; c‑Maf inducing protein inhibits cofilin‑1 activity and alters podocyte cytoskeleton organization.; Authors - Lixia Yu
human	WB,IHC	Mol Med Rep; CMIP is oncogenic in human gastric cancer cells.; Authors - Jianlin Zhang