CMPK1 Monoklonaler Antikörper

CMPK1 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

IF

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

2D11G7

Kat-Nr. : CL594-67519

Synonyme

CMK, CMPK, CMPK1, Cytidine monophosphate kinase, Cytidylate kinase, Deoxycytidylate kinase, UCK, UMK, UMP CMP kinase, UMP CMPK, UMP/CMP kinase, UMP/CMPK, UMPK, Uridine monophosphate kinase

---

Galerie der Validierungsdaten

• 

  IF Staining of HeLa using CL594-67519

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 CMPK1 antibody (CL594-67519, Clone: 2D11G7 ) at dilution of 1:200, CL488-Phalloidin (green).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-67519 bindet in IF CMPK1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CMPK1 fusion protein Ag30084
• Vollständiger Name: cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
• Berechnetes Molekulargewicht: 228 aa, 26 kDa
• Beobachtetes Molekulargewicht: 22-26 kDa
• GenBank-Zugangsnummer: BC014961
• Gene symbol: CMPK1
• Gene ID (NCBI): 51727
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pyrimidine nucleoside monophosphate kinase [UMP/CMP kinase (UMP/CMPK)] (CMPK1) is also named as CMK, CMPK, UCK, UMK, UMPK and belongs to the adenylate kinase family. It plays a crucial role in the formation of UDP, CDP, and dCDP, which are required for cellular nucleic acid synthesis(PMID:11912132). CMPK1 can use a broad spectrum of phosphate donors but the best phosphate donors are ATP and dATP. CMPK1 has been reported as a prognostic marker for non-small cell lung cancer, pancreatic cancer and breast cancer (PMID: 21642870, 22838950, 27558661). The longest deduced protein contains 228 amino acids and has a calculated molecular mass of 26 kD. However, translation likely begins at the second initiation methionine, resulting in a protein of 196 amino acids(PMID:10462544). The shorter form is the endogenously translated protein(PMID:11912132). CMPK1 has 3 isoforms with the molecular mass of 22, 16 and 26 kDa.