CNBP Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CNBP Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 14717-1-AP

Synonyme

CNBP, CNBP1, DM2, PROMM, RNF163, ZCCHC22, Zinc finger protein 9, ZNF9

---

Galerie der Validierungsdaten

• 

  WB analysis of Caco-2 using 14717-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 14717-1-AP (CNBP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14717-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14717-1-AP (CNBP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14717-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14717-1-AP (CNBP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14717-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14717-1-AP (CNBP Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse testis using 14717-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14717-1-AP (CNBP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, HeLa-Zellen, Mausherzgewebe, Maushodengewebe, U-251-Zellen
• Erfolgreiche Detektion in IHC: humanes Eierstockgewebe, humanes Hautgewebe, humanes Herzgewebe, humanes Hirngewebe, humanes Hodengewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Produktinformation

14717-1-AP bindet in WB, IHC, IF, ELISA CNBP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CNBP fusion protein Ag6441
• Vollständiger Name: CCHC-type zinc finger, nucleic acid binding protein
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 20-25 kDa
• GenBank-Zugangsnummer: BC000288
• Gene symbol: CNBP
• Gene ID (NCBI): 7555
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Single stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). Involved in sterol-mediated repression.

Publikationen

Species	Application	Title
human	WB	PLoS Biol; Phosphoglucomutase 1 inhibits hepatocellular carcinoma progression by regulating glucose trafficking.; Authors - Guang-Zhi Jin ; KD Validated
mouse	WB	Mol Cell Proteomics; Quantitative proteomics reveals the essential roles of STIM1 in the testicular cord formation in mouse testis.; Authors - Bo Zheng
mouse	WB,IF	iScience; Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis.; Authors - Qianxing Zou
mouse	WB,IF	Reproduction; Cellular nucleic acid-binding protein is vital to testis development and spermatogenesis in mice.; Authors - Bo Zheng