CNBP Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

CNBP Monoklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

CloneNo.

1H1D12

Rezensionen (1)

Kat-Nr. : 67109-1-Ig

Synonyme

CNBP, CNBP1, DM2, FLJ11631, PROMM, RNF163, ZCCHC22, Zinc finger protein 9, ZNF9

---

• 

  WB analysis using 67109-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67109-1-Ig (CNBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis using 67109-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67109-1-Ig (CNBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67109-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67109-1-Ig (CNBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67109-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67109-1-Ig (CNBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67109-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67109-1-Ig (CNBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 67109-1-Ig

  IP result of anti-CNBP (IP:67109-1-Ig, 5ug; Detection:67109-1-Ig 1:500) with HeLa cells lysate 1600 ug.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, 4T1-Zellen, A549-Zellen, Caco-2-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, HT-29-Zellen, Jurkat-Zellen, K-562-Zellen, NCI-H1299-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IF: See 2 publications below
• IP: See 3 publications below
• RIP: See 1 publications below

Produktinformation

67109-1-Ig bindet in WB, IF, IP, RIP, ELISA CNBP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CNBP fusion protein Ag7114
• Vollständiger Name: CCHC-type zinc finger, nucleic acid binding protein
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 19-25 kDa
• GenBank-Zugangsnummer: BC000288
• Gene symbol: CNBP
• Gene ID (NCBI): 7555
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IP,WB	Nat Microbiol; The SARS-CoV-2 RNA-protein interactome in infected human cells.; Authors - Nora Schmidt ; KO Validated
human	WB,IP	PLoS One; CircPRMT5 promotes progression of osteosarcoma by recruiting CNBP to regulate the translation and stability of CDK6 mRNA; Authors - Yunlu Liu
mouse	WB	J Mol Cell Cardiol; Cellular nucleic acid binding protein facilitates cardiac repair after myocardial infarction by activating β-catenin signaling; Authors - Chong Du
human	WB	PLoS One; circTADA2A inhibited SLC38A1 expression and suppresses melanoma progression through the prevention of CNBP trans-activation; Authors - Longjun Zhang
human,mouse	WB,IP,IF	bioRxiv; A KIF1C-CNBP motor-adaptor complex for trafficking mRNAs to cell protrusions; Authors - Konstadinos Moissoglu ; KO Validated
human,mouse	WB,RIP,IF	Cell Rep; A KIF1C-CNBP motor-adaptor complex for trafficking mRNAs to cell protrusions; Authors - Konstadinos Moissoglu ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Katherine (Verified Customer) (09-25-2023)

The band was clear

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: 293T