CNDP1 Polyklonaler Antikörper

CNDP1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 17759-1-AP

Synonyme

CPGL2, CNDP dipeptidase 1, CN1, Carnosine dipeptidase 1, Beta-Ala-His dipeptidase

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  WB analysis of HEK-293 using 17759-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17759-1-AP (CNDP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 17759-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17759-1-AP (CNDP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 17759-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17759-1-AP (CNDP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 17759-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17759-1-AP (CNDP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17759-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17759-1-AP (CNDP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17759-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17759-1-AP (CNDP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17759-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17759-1-AP (CNDP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17759-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17759-1-AP (CNDP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 17759-1-AP

  Immunofluorescent analysis of A431 cells using 17759-1-AP (CNDP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

17759-1-AP bindet in WB, IHC, IF/ICC, ELISA CNDP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CNDP1 fusion protein Ag12127
• Vollständiger Name: carnosine dipeptidase 1 (metallopeptidase M20 family)
• Berechnetes Molekulargewicht: 508 aa, 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC110295
• Gene symbol: CNDP1
• Gene ID (NCBI): 84735
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beta-Ala-His dipeptidase (CNDP1) encodes the secreted serum carnosinase, which is synthesized in the brain and secreted into the systematic circulation, CNDP1 hydrolyzes L-carnosine and is essential for glucose metabolism and the transportation of activated fatty acids across the mitochondrial membrane (PMID: 32111749, 16046297).

Publikationen

Species	Application	Title
human	IHC	Amino Acids; Serum and urinary carnosinase-1 correlate with kidney function and inflammation; Authors - Shiqi Zhang