CNDP2 Rekombinanter Antikörper

CNDP2 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242373E4

Kat-Nr. : 84924-7-RR

Synonyme

242373E4, CNDP dipeptidase 2, CPGL, Cytosolic non-specific dipeptidase, EC:3.4.13.18

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  WB analysis using 84924-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84924-7-RR (CNDP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat stomach using 84924-7-RR

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 84924-7-RR (CNDP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat stomach using 84924-7-RR

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 84924-7-RR (CNDP2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84924-7-RR

  Biolayer interferometry (BLl) kinetic assays of 84924-7-RR against Human CNDP2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, Mausnierengewebe, Rattennierengewebe, U2OS-Zellen
• Erfolgreiche Detektion in IHC: Ratten-Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84924-7-RR bindet in WB, IHC, ELISA CNDP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CNDP2 fusion protein Ag6684
• Vollständiger Name: CNDP dipeptidase 2 (metallopeptidase M20 family)
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 44-53 kDa
• GenBank-Zugangsnummer: BC001375
• Gene symbol: CNDP2
• Gene ID (NCBI): 55748
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Carnosine dipeptidase II (CNDP2) catalyzes the peptide bond hydrolysis in dipeptides, displaying a non-redundant activity toward threonyl dipeptides. It has high dipeptidase activity toward cysteinyl glycine, an intermediate metabolite in glutathione metabolism (PubMed:19346245, PubMed:12473676). Metabolizes N-lactoyl-amino acids, both through hydrolysis to form lactic acid and amino acids, as well as through their formation by reverse proteolysis (PubMed:25964343). CNDP2 has 2 isoforms with a molecular weight of 53 and 44 kDa.
The isoform 1 is ubiquitously expressed with higher levels in the kidney and liver (at protein level) and expressed in peripheral blood leukocytes (PubMed:12473676). It is also expressed in gastric mucosa and down-regulated in gastric cancer mucosal tissues (at the protein level) (PubMed:24395568). Isoform 2 is broadly expressed in fetal tissues and expressed in the adult liver and placenta. (PMID: 17121880).