CNKSR2 Rekombinanter Antikörper

CNKSR2 Rekombinant Antikörper für Indirect ELISA, Cytometric bead array, WB

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

Indirect ELISA, Cytometric bead array, WB

Konjugation

Unkonjugiert

CloneNo.

240350D6

Kat-Nr. : 83354-3-PBS

Synonyme

KSR2, KIAA0902, Connector enhancer of KSR 2, CNK2, CNK homolog protein 2

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  WB analysis using 83354-3-RR (same clone as 83354-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83354-3-RR (CNKSR2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83354-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 83354-3-RR (same clone as 83354-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83354-3-RR (CNKSR2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83354-3-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 83354-3-RR (same clone as 83354-3-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug CNKSR2 Recombinant antibody (83354-3-RR, Clone:240350D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83354-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00389-1

  Cytometric bead array standard curve of MP00389-1, CNKSR2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83354-2-PBS. Detection antibody: 83354-3-PBS. Standard: Ag21174. Range: 3.125-100ng/mL.

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  Affinity and Kinetic Characterization of 83354-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83354-3-RR against Human CNKSR2 were performed. The affinity constant is 11.1 nM.

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Geprüfte Anwendungen

Produktinformation

83354-3-PBS bindet in Indirect ELISA, Cytometric bead array, WB CNKSR2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CNKSR2 fusion protein Ag21174
• Vollständiger Name: connector enhancer of kinase suppressor of Ras 2
• Berechnetes Molekulargewicht: 1034 aa, 118 kDa
• GenBank-Zugangsnummer: BC126121
• Gene symbol: CNKSR2
• Gene ID (NCBI): 22866
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.