CNKSR2 Rekombinanter Antikörper

CNKSR2 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240350D6

Kat-Nr. : 83354-3-RR

Synonyme

KSR2, KIAA0902, Connector enhancer of KSR 2, CNK2, CNK homolog protein 2

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  WB analysis using 83354-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83354-3-RR (CNKSR2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83354-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83354-3-RR (CNKSR2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of Jurkat using 83354-3-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug CNKSR2 Recombinant antibody (83354-3-RR, Clone:240350D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83354-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83354-3-RR against Human CNKSR2 were performed. The affinity constant is 11.1 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Jurkat-Zellen, MDA-MB-231-Zellen, Maushirngewebe, U-251-Zellen
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83354-3-RR bindet in WB, FC (Intra), ELISA CNKSR2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CNKSR2 fusion protein Ag21174
• Vollständiger Name: connector enhancer of kinase suppressor of Ras 2
• Berechnetes Molekulargewicht: 1034 aa, 118 kDa
• Beobachtetes Molekulargewicht: 118 kDa, 102 kDa
• GenBank-Zugangsnummer: BC126121
• Gene symbol: CNKSR2
• Gene ID (NCBI): 22866
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CNKSR2 is a multidomain protein that functions as a scaffold protein to mediate the mitogen-activated protein kinase pathways downstream from Ras. This gene product is induced by vitamin D and inhibits apoptosis in certain cancer cells. It may also play a role in ternary complex assembly of synaptic proteins at the postsynaptic membrane and coupling of signal transduction to membrane/cytoskeletal remodeling.