CNN2 Monoklonaler Antikörper

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CNN2 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G10F5

Kat-Nr. : 66244-1-PBS

Synonyme

1G10F5, calponin 2, calponin2, Calponin-2, CNN 2

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  WB analysis of HeLa using 66244-1-Ig (same clone as 66244-1-PBS)

  WB result of CNN2 antibody (66244-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNN2 transfected HeLa cells. This data was developed using the same antibody clone with 66244-1-PBS in a different storage buffer formulation.

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  WB analysis using 66244-1-Ig (same clone as 66244-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66244-1-Ig (CNN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66244-1-PBS in a different storage buffer formulation.

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  WB analysis of pig stomach using 66244-1-Ig (same clone as 66244-1-PBS)

  pig stomach tissue were subjected to SDS PAGE followed by western blot with 66244-1-Ig (CNN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66244-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-1080 using 66244-1-Ig (same clone as 66244-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 66244-1-Ig (CNN2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66244-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 66244-1-Ig (same clone as 66244-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66244-1-Ig (CNN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66244-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66244-1-Ig (same clone as 66244-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66244-1-Ig (CNN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66244-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast hyperplasia using 66244-1-Ig (same clone as 66244-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66244-1-Ig (CNN2 Antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66244-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast hyperplasia using 66244-1-Ig (same clone as 66244-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66244-1-Ig (CNN2 Antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66244-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66244-1-PBS bindet in WB, IHC, Indirect ELISA CNN2 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CNN2 fusion protein Ag23594
• Vollständiger Name: calponin 2
• Berechnetes Molekulargewicht: 309 aa, 34 kDa
• Beobachtetes Molekulargewicht: 34-36 kDa
• GenBank-Zugangsnummer: BC141818
• Gene symbol: CNN2
• Gene ID (NCBI): 1265
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). Calponin 1 and calponin 2 are predominately expressed in smooth muscle cells and cardiac muscle cells, respectively. Calponin 3 is highly expressed in many tissues including articular cartilage and brain.