CNNM4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CNNM4 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14066-1-PBS

Synonyme

ACDP4, Ancient conserved domain-containing protein 4, cyclin M4, Cyclin-M4, KIAA1592

---

• 

  WB analysis of HEK-293 using 14066-1-AP (same clone as 14066-1-PBS)

  WB result of CNNM4 antibody (14066-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNNM4 transfected HEK-293 cells. This data was developed using the same antibody clone with 14066-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14066-1-AP (same clone as 14066-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14066-1-AP (CNNM4 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14066-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse testis using 14066-1-AP (same clone as 14066-1-PBS)

  IP result of anti-CNNM4 (IP:14066-1-AP, 4ug; Detection:14066-1-AP 1:1000) with mouse testis tissue lysate 4400ug. This data was developed using the same antibody clone with 14066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 14066-1-AP (same clone as 14066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14066-1-AP (CNNM4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14066-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 14066-1-AP (same clone as 14066-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CNNM4 antibody (14066-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 14066-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

14066-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA CNNM4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CNNM4 fusion protein Ag5121
• Vollständiger Name: cyclin M4
• Berechnetes Molekulargewicht: 87 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC063295
• Gene symbol: CNNM4
• Gene ID (NCBI): 26504
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ancient conserved domain-containing protein 4/cyclin M4 (CNNM4), a membrane protein that stimulates Mg2+ efflux, binds phosphatase of regenerating liver (PRL), which is frequently overexpressed in malignant human cancers (PMID: 25347473). CNNM4 is predominant in the brain, bone marrow, immune system, and especially abundant in the intestinal tract. CNNM4 localizes at the basolateral membrane of the intestinal epithelia and mediates transcellular Mg2+ transport (PMID: 27006114).