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  • KD/KO Validated

CNOT1 Polyklonaler Antikörper

CNOT1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, chIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(85)

Kat-Nr. : 14276-1-AP

Synonyme

AD 005, AD-005, CCR4-associated factor 1, CCR4-NOT transcription complex subunit 1, CDC39



Geprüfte Anwendungen

Erfolgreiche Detektion in WBhumanes Hirngewebe, HEK-293-Zellen, HeLa-Zellen, Maushirngewebe, Mausherzgewebe, Mausnierengewebe, Mausmilzgewebe, Maus-Thymusgewebe
Erfolgreiche IPMausnierengewebe, HeLa-Zellen
Erfolgreiche Detektion in IHChumanes Herzgewebe, humanes Nierengewebe, humanes Milzgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHEK-293-Zellen, sodium arsenite treated HeLa cells

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

14276-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, chIP, RIP, ELISA CNOT1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CNOT1 fusion protein Ag5623
Vollständiger Name CCR4-NOT transcription complex, subunit 1
Berechnetes Molekulargewicht 267 kDa
Beobachtetes Molekulargewicht 240-250 kDa, 174 kDa
GenBank-ZugangsnummerBC040523
Gene symbol CNOT1
Gene ID (NCBI) 23019
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CNOT1 is a component of CCR4-NOT protein complex, which is a regulator of RNA polymerase II transcription, acts as a transcription repressor. CCR4-NOT complex could participate in transcription as well as mRNA degradation. It's highly expressed in brain, heart, thymus, but weak in skeletal muscle and colon. CNOT1 undergoes alternative splicing to produce four isoforms.This is a rabbit polyclonal antibody raised against part chain of C-terminal CNOT1 of human origin. CNOT1 exsits as many isoforms and molecular weight of isoforms are 267, 241 and 173 kDa.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for CNOT1 antibody 14276-1-APProtokoll herunterladen
IHC protocol for CNOT1 antibody 14276-1-APProtokoll herunterladenl
IF protocol for CNOT1 antibody 14276-1-APProtokoll herunterladen
IP protocol for CNOT1 antibody 14276-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Nature

DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs.

Authors - Masashi Yamaji
humanWB

Cell

Roquin promotes constitutive mRNA decay via a conserved class of stem-loop recognition motifs.

Authors - Leppek Kathrin K
mouseWB

Cell

The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.

Authors - Deniz Simsek
humanWB

Nat Cancer

SULT1A1-dependent sulfonation of alkylators is a lineage-dependent vulnerability of liver cancers

Authors - Lei Shi
WB

Mol Cell

An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation

Authors - Stephanie Pei Tung Yiu
humanWB

Mol Cell

Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation

Authors - Markus Höpfler
  • KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Aditya (Verified Customer) (06-20-2024)

Exactly how I would expect an antibodty to behave. Excellent. There is a nonspecific band at 100KDa, but this is probably some variant of CNOT1, so cant fault this at all.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: hek293
FH

Roy (Verified Customer) (06-12-2024)

It does detect CNOT1 by WB from HeLa extracts ( 1/1000 dilution - Overnight incubation at 4°C). It works very well to Immunoprecipitate CNOT1 from both cytoplasmic and nuclear fractions of HeLa cells (4µg /IP)

  • Applications: Western Blot, Immunoprecipitation
  • Primary Antibody Dilution: 1/1000 (WB)
  • Cell Tissue Type: HeLa
FH

aditya (Verified Customer) (10-25-2022)

This is brilliant. We use this for cnot1 pull downs and then use a seperate cnot1 antibody to image on the western. Great for identifying interacting partners.

  • Applications: Western Blot, Protein purification
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Hek293
FH

Tobias (Verified Customer) (10-25-2022)

  • Applications: Western Blot, Immunoprecipitation
FH

Parisa (Verified Customer) (10-17-2022)

good quality, high sensitivity

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hek293
FH

Will (Verified Customer) (01-31-2019)

good antibody, would use again

  • Applications: Western Blot,