CNOT1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

CNOT1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

1A10A11

Rezensionen (2)

Kat-Nr. : 66507-1-Ig

Synonyme

1A10A11, AD 005, AD-005, CCR4-associated factor 1, CCR4-NOT transcription complex subunit 1

---

• 

  WB analysis of HeLa using 66507-1-Ig

  WB result of CNOT1 antibody (66507-1-Ig; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNOT1 transfected HeLa cells.

• 

  WB analysis using 66507-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66507-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66507-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human cerebellum using 66507-1-Ig

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig kidney using 66507-1-Ig

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 66507-1-Ig

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 66507-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66507-1-Ig (CNOT1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 66507-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66507-1-Ig (CNOT1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of HepG2 using 66507-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66507-1-Ig (CNOT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen, A549-Zellen, HEK-293-Zellen, HeLa-Zellen, humanes Cerebellum-Gewebe, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, Mausnierengewebe, NIH/3T3-Zellen, Hausschwein-Nierengewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

66507-1-Ig bindet in WB, IHC, IF/ICC, ELISA CNOT1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CNOT1 fusion protein Ag5623
• Vollständiger Name: CCR4-NOT transcription complex, subunit 1
• Berechnetes Molekulargewicht: 267 kDa
• Beobachtetes Molekulargewicht: 240-250 kDa
• GenBank-Zugangsnummer: BC040523
• Gene symbol: CNOT1
• Gene ID (NCBI): 23019
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CNOT1 is a component of CCR4-NOT protein complex, which is a regulator of RNA polymerase II transcription, acts as a transcription repressor. CCR4-NOT complex could participate in transcription as well as mRNA degradation. It's highly expressed in brain, heart, thymus, but weak in skeletal muscle and colon. CNOT1 undergoes alternative splicing to produce four isoforms. CNOT1 exsits as many isoforms. The isoform one is about 267 kDa and the isoform four is about 173 kDa.

Publikationen

Species	Application	Title
mouse	WB	Mol Cell; Argonaute-miRNA Complexes Silence Target mRNAs in the Nucleus of Mammalian Stem Cells.; Authors - Aishe A Sarshad ; KD Validated
human	WB	Nat Commun; TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.; Authors - Roy Matkovic
mouse	WB,IF	Cell Rep; The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation; Authors - Nicolas Curdy

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Aditya (Verified Customer) (11-01-2024)

It was alright, seems to be good for hrp but not the best with flourescence

• Applications: Western Blot

FH

Roy (Verified Customer) (06-12-2024)

Great antibody to detect CNOT1 by WB from HeLa nuclear and cytoplasmic extracts (1/1000 - Overnight incubation at 4°C). Does also detect the cytoplasmic CNOT1 by immunofluorescence (1/250) in PFA 4% fixed HeLa cells.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1/1000 (WB) - 1/250 (IF)
• Cell Tissue Type: HeLa