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CNOT3 Monoklonaler Antikörper

CNOT3 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

2E12C6

Kat-Nr. : 68436-1-Ig

Synonyme

CCR4 associated factor 3, CNOT3, KIAA0691, LENG2, NOT3, NOT3H

Filter:
  • Western Blot (WB) analysis of various lysates using CNOT3 Monoclonal antibody (68436-1-Ig)

    WB analysis using 68436-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68436-1-Ig (CNOT3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Maushirngewebe, NIH/3T3-Zellen, Hausschwein-Hirngewebe, Kaninchenhirngewebe, Rattenhirngewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

68436-1-Ig bindet in WB, ELISA CNOT3 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Kaninchen, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen CNOT3 fusion protein Ag33646
Vollständiger Name CCR4-NOT transcription complex, subunit 3
Berechnetes Molekulargewicht 120 kDa
Beobachtetes Molekulargewicht120 kDa
GenBank-ZugangsnummerBC016474
Gene symbol CNOT3
Gene ID (NCBI) 4849
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The CCR4-NOT complex, the major deadenylase in mammalian cells, shortens the mRNA poly(A) tail and leads to the destabilization of mRNAs. The CCR4-NOT complex plays pivotal roles in various physiological functions, including cell apoptosis, proliferation, and metabolism [PMID:22342980]. CNOT3, belongs to the CCR4-NOT complex, has a critical role in maintaining mouse and human ESC identity as a protein complex and inhibit differentiation into the extraembryonic lineages. It is also involved in the regulation of the spindle assembly checkpoint [PMID:22342980,22367759].

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for CNOT3 antibody 68436-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Aditya (Verified Customer) (06-20-2024)

Excellent antibody, works significantly better with HRP than with flourescence

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: hek293