• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CNOT4 Monoklonaler Antikörper

CNOT4 Monoklonal Antikörper für IF/ICC, IF-P

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC, IF-P

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4H5H3

Kat-Nr. : CL488-67798

Synonyme

E3 ubiquitin-protein ligase CNOT4, CLONE243, CCR4-NOT transcription complex subunit 4, CCR4-associated factor 4, CCR4 associated factor 4

Filter:
  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CoraLite® Plus 488-conjugated CNOT4 Monoclonal ant (CL488-67798)

    IF Staining of human colon cancer using CL488-67798

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 CNOT4 antibody (CL488-67798, Clone: 4H5H3 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CoraLite® Plus 488-conjugated CNOT4 Monoclonal ant (CL488-67798)

    IF Staining of human colon cancer using CL488-67798

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 CNOT4 antibody (CL488-67798, Clone: 4H5H3 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated CNOT4 Monoclonal ant (CL488-67798)

    IF Staining of HepG2 using CL488-67798

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CNOT4 antibody (CL488-67798, Clone: 4H5H3 ) at dilution of 1:800.

Geprüfte Anwendungen

Erfolgreiche Detektion in IF-Phumanes Kolonkarzinomgewebe
Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67798 bindet in IF/ICC, IF-P CNOT4 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen CNOT4 fusion protein Ag29964
Vollständiger Name CCR4-NOT transcription complex, subunit 4
Berechnetes Molekulargewicht 575 aa, 64 kDa
GenBank-ZugangsnummerBC035590
Gene symbol CNOT4
Gene ID (NCBI) 4850
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CNOT4, also known as CCR4-NOT transcription complex subunit 4 , has E3 ubiquitin ligase activity, and promotes ubiquitination and degradation of target proteins. CNOT4 is involved in activation of the JAK/STAT pathway. CNOT4 exist many isoforms and the range of its molecular weight from 48 to 84 kDa.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for CL Plus 488 CNOT4 antibody CL488-67798Protokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen