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CNOT7 Polyklonaler Antikörper
CNOT7 Polyklonal Antikörper für WB, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 14102-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Maushodengewebe |
| Erfolgreiche IP | Maushodengewebe |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IP | See 1 publications below |
Produktinformation
14102-1-AP bindet in WB, IP, ELISA CNOT7 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Maus |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | CNOT7 fusion protein Ag5235 |
| Vollständiger Name | CCR4-NOT transcription complex, subunit 7 |
| Berechnetes Molekulargewicht | 285 aa, 33 kDa |
| Beobachtetes Molekulargewicht | 33-35 kDa |
| GenBank-Zugangsnummer | BC060852 |
| Gene symbol | CNOT7 |
| Gene ID (NCBI) | 29883 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Cnot7, Ccr4-Not complex 7, is a component of CCR4-NOT complex and known to be a transcription factor and a modulator of mRNA degradation in yeast. Mammalian Cnot7 interacts with Tob and its family members, which is known as an inhibitor of BMP signaling through interfering with the Smad system and is involved in bone metabolism [PMID:17451368]. It is one of at least 8 subunits that make up the CCR4-Not transcription complex, which is involved in negative regulation of the pol II directed transcription in yeast24 and may be involved inglobal gene regulation by modulation of TATAA-binding protein (TBP) activity [PMID:10880468].
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for CNOT7 antibody 14102-1-AP | Protokoll herunterladen |
| IP protocol for CNOT7 antibody 14102-1-AP | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Nature DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs. | ||
Mol Cell Argonaute-miRNA Complexes Silence Target mRNAs in the Nucleus of Mammalian Stem Cells. | ||
Nat Commun TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV. | ||
Open Biol RNA binding protein AUF1/HNRNPD regulates nuclear export, stability and translation of SNCA transcripts | ||
J Inflamm (Lond) The functional characterization of phosphorylation of tristetraprolin at C-terminal NOT1-binding domain.
| ||
Int J Mol Med B cell translocation gene 1 reduces the biological outcome of kidney cancer through induction of cell proliferation, cell cycle arrest, cell apoptosis and cell metastasis. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Roy (Verified Customer) (06-13-2024) | Works well to detect CNOT7 (validated through siRNA-mediated KD of CNOT7 - 1/1000 dilution with an overnight incubation at 4°C).
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