CNOT8 Polyklonaler Antikörper

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• KD/KO Validated

CNOT8 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10752-1-PBS

Synonyme

CALIF, CAF2, CAF1-like protein, Caf1b, CAF1 like protein

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  WB analysis of Jurkat using 10752-1-AP (same clone as 10752-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10752-1-AP (CNOT8 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10752-1-AP (same clone as 10752-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10752-1-AP (CNOT8 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 10752-1-AP (same clone as 10752-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10752-1-AP (CNOT8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 10752-1-AP (same clone as 10752-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10752-1-AP (CNOT8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 10752-1-AP (same clone as 10752-1-PBS)

  IP result of anti-CNOT8 (IP:10752-1-AP, 4ug; Detection:10752-1-AP 1:600) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 10752-1-AP (same clone as 10752-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10752-1-AP (CNOT8 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 10752-1-AP (same clone as 10752-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10752-1-AP (CNOT8 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 10752-1-AP (same clone as 10752-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10752-1-AP (CNOT8 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 10752-1-AP (same clone as 10752-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10752-1-AP (CNOT8 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 10752-1-AP (same clone as 10752-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10752-1-AP (CNOT8 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10752-1-AP (same clone as 10752-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CNOT8 antibody (10752-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10752-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA CNOT8 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CNOT8 fusion protein Ag1201
• Vollständiger Name: CCR4-NOT transcription complex, subunit 8
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC017366
• Gene symbol: CNOT8
• Gene ID (NCBI): 9337
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCR4-NOT transcription complex subunit 8(CNOT8), is a ubiquitous transcription factor, which required for a diverse set of processes. The CCR4-NOT complex is a global regulator of RNA polymerase II, and functions as general transcription regulation complex. It consisted part by CCR4, NOT1 to NOT5, and CAF1. This is a rabbit polyclonal antibody raised against the full-length chain of human CNOT8.