CNPY2/MSAP Polyklonaler Antikörper

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• KD/KO Validated

CNPY2/MSAP Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14635-1-PBS

Synonyme

CNPY2, CNPY2, MSAP, CNPY2,MSAP, canopy 2 homolog (zebrafish), HP10390

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  WB analysis of mouse pancreas using 14635-1-AP (same clone as 14635-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14635-1-AP (CNPY2,MSAP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.

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  WB analysis using 14635-1-AP (same clone as 14635-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14635-1-AP (CNPY2, MSAP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 14635-1-AP (same clone as 14635-1-PBS)

  WB result of CNPY2, MSAP antibody (14635-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPY2, MSAP transfected HepG2 cells. This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 14635-1-AP (same clone as 14635-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 14635-1-AP (CNPY2,MSAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse pancreas using 14635-1-AP (same clone as 14635-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14635-1-AP (CNPY2,MSAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 14635-1-AP (same clone as 14635-1-PBS)

  IP result of anti-CNPY2, MSAP (IP:14635-1-AP, 3ug; Detection:14635-1-AP 1:500) with HepG2 cells lysate 600ug. This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 14635-1-AP (same clone as 14635-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14635-1-AP (CNPY2, MSAP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 14635-1-AP (same clone as 14635-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14635-1-AP (CNPY2, MSAP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 14635-1-AP (same clone as 14635-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14635-1-AP (CNPY2,MSAP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 14635-1-AP (same clone as 14635-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14635-1-AP (CNPY2,MSAP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14635-1-AP (same clone as 14635-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 14635-1-AP (CNPY2,MSAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14635-1-AP (same clone as 14635-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CNPY2, MSAP antibody (14635-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 14635-1-AP (same clone as 14635-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human CNPY2, MSAP (14635-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14635-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA CNPY2/MSAP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CNPY2/MSAP fusion protein Ag6214
• Vollständiger Name: canopy 2 homolog (zebrafish)
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 17-21 kDa
• GenBank-Zugangsnummer: BC065015
• Gene symbol: CNPY2
• Gene ID (NCBI): 10330
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth. It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 can stabilize MRLC and thus bring about an increase in neurite outgrowth. (PMID: 12826659 ) This antibody is specific to CNPY2.