CNPY3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CNPY3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 15215-1-AP

Synonyme

Expanded repeat-domain protein CAG/CTG 5, ERDA5, CTG4A, CTG repeat protein 4a, canopy 3 homolog (zebrafish)

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  WB analysis of human brain using 15215-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15215-1-AP (CNPY3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15215-1-AP

  WB result of CNPY3 antibody (15215-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPY3 transfected A549 cells.

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  WB analysis using 15215-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15215-1-AP (CNPY3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15215-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15215-1-AP (CNPY3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 15215-1-AP

  IP result of anti-CNPY3 (IP:15215-1-AP, 3ug; Detection:15215-1-AP 1:500) with mouse kidney tissue lysate 6000ug.

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  IHC staining of human colon cancer using 15215-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15215-1-AP (CNPY3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15215-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15215-1-AP (CNPY3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 15215-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CNPY3 antibody (15215-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen, humanes Hirngewebe, Jurkat-Zellen
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below

Produktinformation

15215-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CNPY3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CNPY3 fusion protein Ag7382
• Vollständiger Name: canopy 3 homolog (zebrafish)
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 30-40 kDa
• GenBank-Zugangsnummer: BC004423
• Gene symbol: CNPY3
• Gene ID (NCBI): 10695
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IHC	Cell Oncol (Dordr); The SLITRK4-CNPY3 axis promotes liver metastasis of gastric cancer by enhancing the endocytosis and recycling of TrkB in tumour cells; Authors - Yao-Qi Zhou
human	WB,IHC	Mol Med; CNPY3's regulation of tumor microenvironment and its impact on colon cancer aggressiveness; Authors - Xucan Gao
human	WB	J Neuroimmunol; A novel rare variant of CNPY3 from familial NMOSD impairs the TLR-mediated immune response; Authors - Yongxin Mo
human	WB	Stem Cell Res; Generation of CNPY3 knock out cell line in the H1 (WA01) hESC background; Authors - Xing Qi ; KO Validated
human	WB,IHC	World J Gastrointest Oncol; Canopy FGF signaling regulator 3 affects prognosis, immune infiltration, and PI3K/AKT pathway in colon adenocarcinoma; Authors - Xu-Can Gao