Cannabinoid receptor 1 Polyklonaler Antikörper

Cannabinoid receptor 1 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(29)

Kat-Nr. : 17978-1-AP

Synonyme

CNR1, CANN6, cannabinoid receptor 1 (brain), CB R, CB1

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  WB analysis using 17978-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17978-1-AP (Cannabinoid receptor 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17978-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17978-1-AP (Cannabinoid receptor 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17978-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17978-1-AP (Cannabinoid receptor 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 17978-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17978-1-AP (Cannabinoid receptor 1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17978-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17978-1-AP (Cannabinoid receptor 1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 17978-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Cannabinoid receptor 1 antibody (17978-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 22 publications below
• IHC: See 5 publications below
• IF: See 5 publications below

Produktinformation

17978-1-AP bindet in WB, IHC, IF-P, ELISA Cannabinoid receptor 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cannabinoid receptor 1 fusion protein Ag12371
• Vollständiger Name: cannabinoid receptor 1 (brain)
• Berechnetes Molekulargewicht: 472 aa, 53 kDa
• Beobachtetes Molekulargewicht: 45 kDa, 66 kDa
• GenBank-Zugangsnummer: BC074812
• Gene symbol: Cannabinoid receptor 1
• Gene ID (NCBI): 1268
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cannabinoid receptor 1 (CNR1, or CB1) and CNR2 (CB2) are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family. The CB1 receptor is expressed mainly in the brain. The CB2 receptor is expressed mainly in the immune system and in hematopoietic cells. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana.

Publikationen

Species	Application	Title
mouse	WB	Elife; p21-activated kinase 1 restricts tonic endocannabinoid signaling in the hippocampus.; Authors - Shuting Xia
mouse	WB	Transl Psychiatry; Deletion of Fgf14 confers resilience to basal and stress-induced depressive-like behavior and reduces anxiety in mice; Authors - Francesca Montarolo
mouse	WB	Chin Med; Electroacupuncture repairs intestinal barrier by upregulating CB1 through gut microbiota in DSS-induced acute colitis; Authors - Jingze Yang
mouse,human	WB,IF	Neuropharmacology; Δ9-tetrahydrocannabinol induces blood-brain barrier disruption: Involving the activation of CB1R and oxidative stress; Authors - Qianyao Zhang
mouse	WB	Hum Mol Genet; BDNF overexpression prevents cognitive deficit elicited by adolescent cannabis exposure and host susceptibility interaction.; Authors - Hadar Segal-Gavish
rat	IHC	Phytomedicine; Endocannabinoid signalling/cannabinoid receptor 2 is involved in icariin-mediated protective effects against bleomycin-induced pulmonary fibrosis.; Authors - Wenjing Du