CNTN1 Polyklonaler Antikörper

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• KD/KO Validated

CNTN1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 13843-1-AP

Synonyme

CNTN1, contactin 1, F3, Glycoprotein gp135, GP135, Neural cell surface protein F3

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Galerie der Validierungsdaten

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  WB analysis using 13843-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13843-1-AP (CNTN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 13843-1-AP

  IP Result of anti-CNTN1 (IP:13843-1-AP, 4ug; Detection:13843-1-AP 1:1500) with rat brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 13843-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13843-1-AP (CNTN1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13843-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13843-1-AP (CNTN1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maus-Cerebellum-Gewebe, Ratten-Cerebellum-Gewebe, Rattenhirngewebe
• Erfolgreiche IP: Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

13843-1-AP bindet in WB, IP, IHC, IF, ELISA CNTN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CNTN1 fusion protein Ag4803
• Vollständiger Name: contactin 1
• Berechnetes Molekulargewicht: 1018 aa, 113 kDa
• Beobachtetes Molekulargewicht: 135 kDa
• GenBank-Zugangsnummer: BC036569
• Gene symbol: CNTN1
• Gene ID (NCBI): 1272
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Contactin-1 (CNTN1), also known as Glycoprotein gp135 or Neural cell surface protein F3, is a neuronal cell adhesion molecule of the immunoglobulin superfamily (PMID: 3049624). It consists of six Ig domains, four fibronectin-like motifs, and a glycosylphosphatidylinositol (GPI)-moiety. Expressed by the central and peripheral neurons, CNTN1 contributes to the formation and function of neuronal connections. It plays a role in axon extension and formation of septate-like junctions between axons and myelinating glial cells (PMID: 23724143). In addition, acting as a ligand for Notch, CNTN1 initiates a NOTCH/DTX1 signaling pathway that promotes oligodendrocyte maturation and myelination (PMID: 14567914).

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.; Authors - I-Ling Lu ; KD Validated
rat	WB	Nat Commun; Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination.; Authors - Y Poitelon
human	IHC	Ann Clin Transl Neurol; CIDP/autoimmune nodopathies with nephropathy: a case series study; Authors - Yuwei Tang
mouse	IF,WB	Front Mol Neurosci; Contactin-1/F3 Regulates Neuronal Migration and Morphogenesis Through Modulating RhoA Activity.; Authors - Yi-An Chen ; KD Validated
human	WB	Front Genet; CNTN-1 Upregulation Induced by Low-Dose Cisplatin Promotes Malignant Progression of Lung Adenocarcinoma Cells via Activation of Epithelial-Mesenchymal Transition.; Authors - Ruijie Zhang
human	WB	Arch Med Sci; CNTN-1 promotes docetaxel resistance and epithelial-to-mesenchymal transition via the PI3K/Akt signaling pathway in prostate cancer.; Authors - Binshen Chen

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