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COA4 Polyklonaler Antikörper

COA4 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30721-1-PBS

Synonyme

CHCHD8, E2IG2, E2 induced gene 2 protein

Filter:
  • Western Blot (WB) analysis of various lysates using COA4 Polyclonal antibody (30721-1-AP)

    WB analysis using 30721-1-AP (same clone as 30721-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 30721-1-AP (COA4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30721-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using COA4 Polyclonal antibody (30721-1-AP)

    WB analysis using 30721-1-AP (same clone as 30721-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30721-1-AP (COA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30721-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using COA4 Polyclonal antibody (30721-1-AP)

    IHC staining of human ovary cancer using 30721-1-AP (same clone as 30721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30721-1-AP (COA4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30721-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

30721-1-PBS bindet in WB, IHC, Indirect ELISA COA4 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen COA4 fusion protein Ag33966
Vollständiger Name Cytochrome c oxidase assembly factor 4 homolog, mitochondrial
Berechnetes Molekulargewicht10 kDa
Beobachtetes Molekulargewicht12 kDa
GenBank-ZugangsnummerNM_016565.2
Gene symbol COA4
Gene ID (NCBI) 51287
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The coiled-coil-helix-coiled-coil-helix domain containing 8 (CHCHD8) is a putative COX assembly factor known as cytochrome c oxidase assembly factor 4 homolog (CoA4). COA4 is a newly identified CcO assembly factor which is a twin CX(9)C motif mitochondrial protein localized in the intermembrane space linked with the inner membrane of mitochondria. Its transport into intermembrane space depends on the MIA40 trans-site receptor machinery. (PMID: 37345060)