COA6 Polyklonaler Antikörper

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• KD/KO Validated

COA6 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24209-1-PBS

Synonyme

C1orf31

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  WB analysis of HEK-293 using 24209-1-AP (same clone as 24209-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24209-1-AP (COA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24209-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 24209-1-AP (same clone as 24209-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24209-1-AP (COA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24209-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 24209-1-AP (same clone as 24209-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24209-1-AP (COA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24209-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 24209-1-AP (same clone as 24209-1-PBS)

  IP result of anti-COA6 (IP:24209-1-AP, 4ug; Detection:24209-1-AP 1:500) with HEK-293 cells lysate 1760ug. This data was developed using the same antibody clone with 24209-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 24209-1-AP (same clone as 24209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24209-1-AP (COA6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24209-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 24209-1-AP (same clone as 24209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24209-1-AP (COA6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24209-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 24209-1-AP (same clone as 24209-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C1orf31 antibody (24209-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24209-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

24209-1-PBS bindet in WB, IHC, IP, Indirect ELISA COA6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COA6 fusion protein Ag21525
• Vollständiger Name: chromosome 1 open reading frame 31
• Berechnetes Molekulargewicht: 125 aa, 14 kDa
• Beobachtetes Molekulargewicht: 10 - 18 kDa
• GenBank-Zugangsnummer: BC116455
• Gene symbol: COA6
• Gene ID (NCBI): 388753
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C1orf31, also known as COA6, is a conserved mitochondrial protein required for respiratory complex IV biogenesis. Recently study reported that C1orf31 is required for maintenance of cytochrome c oxidase (CcO) subunit levels, and has role in the Cu delivery pathway to CcO. Defects in C1orf31 is associated with mitochondrial respiratory chain disease (MRCD). (24549041)