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Collagen Type XII Polyklonaler Antikörper

Collagen Type XII Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30045-1-PBS

Synonyme

COL12, COL12A1, COL12A1L, Collagen alpha 1(XII) chain, Collagen alpha-1(XII) chain

Filter:
  • Western Blot (WB) analysis of various lysates using Collagen Type XII Polyclonal antibody (30045-1-AP)

    WB analysis using 30045-1-AP (same clone as 30045-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30045-1-AP (COL12A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30045-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using Collagen Type XII Polyclonal antibody (30045-1-AP)

    IF Staining of U2OS using 30045-1-AP (same clone as 30045-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using COL12A1 antibody (30045-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 30045-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

30045-1-PBS bindet in WB, IF/ICC, Indirect ELISA Collagen Type XII und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Collagen Type XII fusion protein Ag32602
Vollständiger Name collagen, type XII, alpha 1
Berechnetes Molekulargewicht333kd
Beobachtetes Molekulargewicht310-333 kDa
GenBank-ZugangsnummerNM_004370
Gene symbol COL12A1
Gene ID (NCBI) 1303
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COL12A1 (Collagen alpha-1(XII) chain), also known as COL12A1L. It is predicted to be located in the ECM. Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix. The molecular weight of COL12A1 is 333 kDa.