Collagen Type XVI Polyklonaler Antikörper

Collagen Type XVI Polyklonal Antikörper für WB, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 19802-1-AP

Synonyme

COL16A1, COL16, FP1572, Collagen alpha-1(XVI) chain, Collagen alpha 1(XVI) chain

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  WB analysis of human placenta using 19802-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19802-1-AP (Collagen Type XVI antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 19802-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19802-1-AP (Collagen Type XVI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse skin using 19802-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Collagen Type XVI antibody (19802-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IF-P: Maushautgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

19802-1-AP bindet in WB, IF-P, ELISA Collagen Type XVI und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: collagen, type XVI, alpha 1
• Berechnetes Molekulargewicht: 158 kDa
• Beobachtetes Molekulargewicht: 158 kDa
• GenBank-Zugangsnummer: NM_001856
• Gene symbol: COL16A1
• Gene ID (NCBI): 1307
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COL16A1, also named as FP1572, belongs to the fibril-associated collagens with interrupted helices (FACIT) family. COL16A1 is involved in mediating cell attachment and inducing integrin-mediated cellular reactions, such as cell spreading and alterations in cell morphology. The antibody is specific to COL16A1.

Publikationen

Species	Application	Title
Human	WB	Nat Commun; TRIP13 promotes error-prone nonhomologous end joining and induces chemoresistance in head and neck cancer.; Authors - Rajat Banerjee
human	WB	Aging (Albany NY); MicroRNA-152 and -181a participate in human dermal fibroblasts senescence acting on cell adhesion and remodeling of the extra-cellular matrix.; Authors - Mancini Mara M