Collagen Type I Rekombinanter Antikörper

Collagen Type I Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240665D2

Kat-Nr. : 83752-3-PBS

Synonyme

COL1, COL1A2, 240665D2, Alpha-2 type I collagen, col I

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  WB analysis of rat skin using 83752-3-RR (same clone as 83752-3-PBS)

  rat skin tissue were subjected to SDS PAGE followed by western blot with 83752-3-RR (Collagen Type I antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83752-3-PBS in a different storage buffer formulation.

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  WB analysis using 83752-3-RR (same clone as 83752-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83752-3-RR (COL1A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83752-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00716-3

  Sandwich ELISA standard curve of MP00716-3, Human Collagen Type I Recombinant Matched Antibody Pair - PBS only. 83752-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag6281. 83752-4-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  Sample test of MP00716-3

  Serum of sixteen individual healthy human donors was measured. The Collagen Type I concentration of detected samples was determined to be 2.67 ng/mL with a range of ND-15.45 ng/mL.

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  Affinity and Kinetic Characterization of 83752-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 83752-3-PBS against Human Collagen Type I was performed. The affinity constant is 47.7 pM.

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  Affinity and Kinetic Characterization of 83752-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83752-3-RR against Human Collagen Type I were performed. The affinity constant is 0.777 nM.

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Geprüfte Anwendungen

Produktinformation

83752-3-PBS bindet in WB, Sandwich ELISA, Indirect ELISA, Sample test Collagen Type I und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Collagen Type I fusion protein Ag6281
• Vollständiger Name: collagen, type I, alpha 2
• Berechnetes Molekulargewicht: 1366 aa, 130 kDa
• Beobachtetes Molekulargewicht: 100-160 kDa
• GenBank-Zugangsnummer: BC054498
• Gene symbol: COL1A2
• Gene ID (NCBI): 1278
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. This antibody raised against 1017-1366 aa of human pro-alpha 2 chain of type l collagen can recognize collagen alpha 2(1) chain and C-terminal propeptide of pro-alpha 2(1) chain.