Collagen Type I Rekombinanter Antikörper

Collagen Type I Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240665D2

Kat-Nr. : 83752-3-RR

Synonyme

COL1, COL1A2, 240665D2, Alpha-2 type I collagen, col I

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  WB analysis of rat skin using 83752-3-RR

  rat skin tissue were subjected to SDS PAGE followed by western blot with 83752-3-RR (Collagen Type I antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83752-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83752-3-RR (COL1A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83752-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83752-3-RR against Human Collagen Type I were performed. The affinity constant is 0.777 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MG-63 cells, Rattenhautgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83752-3-RR bindet in WB, ELISA Collagen Type I und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Collagen Type I fusion protein Ag6281
• Vollständiger Name: collagen, type I, alpha 2
• Berechnetes Molekulargewicht: 1366 aa, 130 kDa
• Beobachtetes Molekulargewicht: 100-160 kDa
• GenBank-Zugangsnummer: BC054498
• Gene symbol: COL1A2
• Gene ID (NCBI): 1278
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. This antibody raised against 1017-1366 aa of human pro-alpha 2 chain of type l collagen can recognize collagen alpha 2(1) chain and C-terminal propeptide of pro-alpha 2(1) chain.