Collagen Type III (N-terminal) Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Collagen Type III (N-terminal) Polyklonal Antikörper für WB, IHC, IF-P, IF-Fro, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (6)

Anwendung

WB, IHC, IF-P, IF-Fro, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(587)

Rezensionen (5)

Kat-Nr. : 22734-1-AP

Synonyme

COL3, COL3A1, Collagen alpha 1(III) chain, Collagen alpha-1(III) chain, Collagen Type III

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• 

  WB analysis of mouse skin using 22734-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skin using 22734-1-AP

  rat skin tissue were subjected to SDS PAGE followed by western blot with 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skin using 22734-1-AP

  IP result of anti-Collagen Type III (N-terminal) (IP:22734-1-AP, 4ug; Detection:22734-1-AP 1:3000) with mouse skin tissue lysate 1120 ug.

• 

  IHC staining of human hepatocirrhosis using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 40x lens).

• 

  IHC staining of human malignant melanoma using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human colon using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 40x lens).

• 

  IHC staining of human skin using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human skin using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 40x lens).

• 

  IHC staining of mouse kidney using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon using 22734-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Collagen Type III (N-terminal) antibody (22734-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human colon using 22734-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Collagen Type III (N-terminal) antibody (22734-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human colon using 22734-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Collagen Type III (N-terminal) antibody (22734-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Cytokeratin 20 antibody (60183-1-Ig, Clone: 4D10A4, Magenta), CoraLite®594 CD68 antibody (CL594-25747, red).

• 

  IF Staining of mouse colon using 22734-1-AP

  Immunofluorescence of mouse colon: Frozen OCT-embedded mouse colon sections were stained with anti-E-cadherin antibody (20874-1-AP) labeled with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2, green) in the frist step,anti-CD45 antibody (80297-1-RR) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, orange) in the second step,anti-Collagen Type III antibody (22734-1-AP) labeled with FlexAble CoraLite647 Kit (KFA003, magenta) in the third step, and DAPI (blue).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushautgewebe, Rattenhautgewebe
• Erfolgreiche IP: Maushautgewebe
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe, humanes Kolongewebe, humanes malignes Melanomgewebe, humanes Pankreaskarzinomgewebe, humanes Hautkrebsgewebe, humanes Hautgewebe, Maus-Kolongewebe, Mausherzgewebe, Mausnierengewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Kolongewebe
• Erfolgreiche Detektion in IF-Fro: Maus-Kolongewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 409 publications below
• IHC: See 169 publications below
• IF: See 107 publications below

Produktinformation

22734-1-AP bindet in WB, IHC, IF-P, IF-Fro, IP, ELISA Collagen Type III (N-terminal) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Hausschwein, Huhn, Hund, Kaninchen, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Collagen Type III (N-terminal) fusion protein Ag18658
• Vollständiger Name: collagen, type III, alpha 1
• Berechnetes Molekulargewicht: 1466 aa, 139 kDa
• Beobachtetes Molekulargewicht: 140-180 kDa
• GenBank-Zugangsnummer: BC028178
• Gene symbol: COL3A1
• Gene ID (NCBI): 1281
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Type III collagen is a fibrillar forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341). This antibody raised against 24-152 aa of prepro α1 (III) chain of human type III procollagen detects type III procollagen at 140-180 kDa and also in some lysates reveals a 70-kDa band which has been reported and may represent a cleaved form of type III procollagen (PMID: 17424834; 19648160; 22802960).

Publikationen

Species	Application	Title
human	IF	Cancer Cell; Single-cell multi-stage spatial evolutional map of esophageal carcinogenesis; Authors - Jiang Chang
rat	WB	Cell Metab; Sphingolipid metabolism controls mammalian heart regeneration; Authors - Xiaoqian Ji
human,mouse	IF	Nat Aging; Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer; Authors - Yifei Cheng
human	WB	Adv Sci (Weinh); LIMA1 O-GlcNAcylation Promotes Hepatic Lipid Deposition through Inducing β-catenin-Regulated FASn Expression in Metabolic Dysfunction-Associated Steatotic Liver Disease; Authors - Fuji Yang
mouse	IHC	Acta Pharm Sin B; SMYD3-PARP16 axis accelerates unfolded protein response and mediates neointima formation.; Authors - Fen Long
		Sci Adv; Designer cellular spheroids with DNA origami for drug screening; Authors - Jiayi Wei

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Guennec (Verified Customer) (10-12-2023)

I have 2 bands at 70 KDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Mouse Heart Tissue

Collagen Type III (N-terminal) Antibody Western Blot validation (1/1000 dilution) in Mouse Heart Tissue (Cat no:22734-1-AP)

FH

Sarah-Eve (Verified Customer) (02-19-2023)

Works well. One band observed at 180 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Human right ventricular fibroblasts

FH

Gayatri (Verified Customer) (06-10-2022)

Good signal but inexplicable molecular weight. Observe bands at 70 kDa instead of expected 140-180 kDa although this product is validated for Rat. Observed in both rat heart and tail lysates.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Rat Heart Tissue

FH

Yuki (Verified Customer) (03-30-2022)

Works well for IF application and presented with intense staining

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: skin cells

Collagen Type III (N-terminal) Antibody Immunohistochemistry validation (1:50 dilution) in skin cells (Cat no:22734-1-AP)

FH

Ryan (Verified Customer) (04-20-2018)

Antibody worked well for IF application and presented with intense staining of both medial and adventitial collagen in artery sections

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: paraffin carotid artery section

Collagen Type III (N-terminal) Antibody Immunofluorescence validation (1:400 dilution) in paraffin carotid artery section (Cat no:22734-1-AP)