Collagen Type III Monoklonaler Antikörper

Collagen Type III Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3B1F3

Kat-Nr. : 68320-1-PBS

Synonyme

COL3, COL3A1, 3B1F3, Collagen alpha 1(III) chain, Collagen alpha-1(III) chain

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  WB analysis using 68320-1-Ig (same clone as 68320-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68320-1-Ig (COL3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 68320-1-Ig (same clone as 68320-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68320-1-Ig (COL3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

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  WB analysis of human rectum using 68320-1-Ig (same clone as 68320-1-PBS)

  human rectum tissue were subjected to SDS PAGE followed by western blot with 68320-1-Ig (COL3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit large intestine using 68320-1-Ig (same clone as 68320-1-PBS)

  rabbit large intestine tissue were subjected to SDS PAGE followed by western blot with 68320-1-Ig (COL3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 68320-1-Ig (same clone as 68320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 68320-1-Ig (same clone as 68320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 68320-1-Ig (same clone as 68320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 68320-1-Ig (same clone as 68320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 68320-1-Ig (same clone as 68320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 68320-1-Ig (same clone as 68320-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using COL3A1 antibody (68320-1-Ig, Clone: 3B1F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68320-1-PBS bindet in WB, IHC, Indirect ELISA Collagen Type III und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Collagen Type III fusion protein Ag4229
• Vollständiger Name: collagen, type III, alpha 1
• Berechnetes Molekulargewicht: 1466 aa, 139 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC028178
• Gene symbol: COL3A1
• Gene ID (NCBI): 1281
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Type III collagen is a fibrillar-forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome type IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341).