COL4A3BP Polyklonaler Antikörper

COL4A3BP Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 15191-1-AP

Synonyme

Ceramide transfer protein, CERT, CERTL, COL4A3BP, GPBP, hCERT, STARD11

---

Galerie der Validierungsdaten

• 

  WB analysis using 15191-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15191-1-AP (COL4A3BP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human pancreas using 15191-1-AP

  human pancreas tissue were subjected to SDS PAGE followed by western blot with 15191-1-AP (COL4A3BP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15191-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15191-1-AP (COL4A3BP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 15191-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15191-1-AP (COL4A3BP Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse pancreas using 15191-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15191-1-AP (COL4A3BP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15191-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15191-1-AP (COL4A3BP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15191-1-AP

  IP Result of anti-COL4A3BP (IP:15191-1-AP, 4ug; Detection:15191-1-AP 1:300) with mouse brain tissue lysate 3680ug.

• 

  IHC staining of human pancreas using 15191-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 15191-1-AP (COL4A3BP Antibody) at dilution of 1:50.

• 

  IHC staining of human pancreas using 15191-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 15191-1-AP (COL4A3BP Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 15191-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 15191-1-AP (COL4A3BP Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 15191-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 15191-1-AP (COL4A3BP Antibody) at dilution of 1:50.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HEK-293-Zellen, humanes Pankreasgewebe, Mausnierengewebe, Maus-Pankreasgewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below

Produktinformation

15191-1-AP bindet in WB, IP, IHC, ELISA COL4A3BP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COL4A3BP fusion protein Ag7277
• Vollständiger Name: collagen, type IV, alpha 3 (Goodpasture antigen) binding protein
• Berechnetes Molekulargewicht: 71 kDa
• Beobachtetes Molekulargewicht: 80-89 kDa
• GenBank-Zugangsnummer: BC000102
• Gene symbol: COL4A3BP
• Gene ID (NCBI): 10087
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COL4A3BP(Collagen type IV alpha-3-binding protein) is also named as CERT, STARD11. CERT extracts ceramide from the ER and carries it to the Golgi apparatus in a non-vesicular manner and that a particularly efficient cycle of CERT movement for trafficking of ceramide may proceed at membrane contact sites between the ER and the Golgi apparatus(PMID:17314061). This protein has 3 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
rat	WB	Acta Biochim Biophys Sin (Shanghai); Exogenous hydrogen sulfide inhibits apoptosis by regulating endoplasmic reticulum stress-autophagy axis and improves myocardial reconstruction after acute myocardial infarction.; Authors - Yaling Li
mouse	WB	Ren Fail; LncTUG1 ameliorates renal tubular fibrosis in experimental diabetic nephropathy through the miR-145-5p/dual-specificity phosphatase 6 axis; Authors - Taoxia Wang
human	WB	Cell Rep; Golgi retention and oncogenic KIT signaling via PLCγ2-PKD2-PI4KIIIβ activation in gastrointestinal stromal tumor cells; Authors - Yuuki Obata ; KD Validated
human,mouse	WB	Arch Biochem Biophys; The ceramide transport protein CERT is involved in alkylacylglycerol transfer from the ER to the Golgi for the biosynthesis of ether phospholipid; Authors - Yasuhiro Horibata
human,mouse	WB	Oncogenesis; Ablation of sphingosine kinase 2 suppresses fatty liver-associated hepatocellular carcinoma via downregulation of ceramide transfer protein; Authors - Xin Tracy Liu