COL4A4 Polyklonaler Antikörper

COL4A4 Polyklonal Antikörper für IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Kat-Nr. : 19674-1-AP

Synonyme

COL4, Collagen Type IV, COL4A 4, collagen, type IV, alpha 4

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  IF Staining of HeLa using 19674-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19674-1-AP (Collagen Type IV antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Produktinformation

19674-1-AP bindet in WB, IHC, IF/ICC, ELISA COL4A4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ente, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: collagen, type IV, alpha 4
• Berechnetes Molekulargewicht: 164 kDa
• GenBank-Zugangsnummer: NM_000092
• Gene symbol: COL4A4
• Gene ID (NCBI): 1286
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COL4A4 belongs to the type IV collagen family. COL4A4 is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A4 are a cause of Alport syndrome autosomal recessive (APSAR). Defects in COL4A4 are a cause of benign familial hematuria (BFH). This antibody is specific to COL4A4.

Publikationen

Species	Application	Title
rat	WB	J Ethnopharmacol; In silico prediction and experimental validation to reveal the protective mechanism of Puerarin against excessive extracellular matrix accumulation through inhibiting ferroptosis in diabetic nephropathy; Authors - Biyu Hou
	WB	PLoS Negl Trop Dis; Mining host candidate regulators of schistosomiasis-induced liver fibrosis in response to artesunate therapy through transcriptomics approach; Authors - Yajie Yuan
rat	WB	Eur J Pharmacol; Sirt1 activation prevents anti-Thy 1.1 mesangial proliferative glomerulonephritis in the rat through the Nrf2/ARE pathway.; Authors - Kaipeng Huang
rat	IF	J Chem Neuroanat; Berberine protects against chronic cerebral hypoperfusion-induced cognitive impairment and hippocampal damage via regulation of the ERK/Nrf2 pathway.; Authors - Ningqun Wang
duck	WB	Poult Sci; Co-exposure of polyvinyl chloride microplastics with cadmium promotes nonalcoholic fatty liver disease in female ducks through oxidative stress and glycolipid accumulation; Authors - Yan Chen
human	IF	Cell; Early rhombic lip Protogenin+ve stem cells in a human-specific neurovascular niche initiate and maintain group 3 medulloblastoma; Authors - Abhirami Visvanathan