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COL5A3 Polyklonaler Antikörper

COL5A3 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30204-1-PBS

Synonyme

COL5, Collagen alpha 3(V) chain, Collagen alpha-3(V) chain, Collagen Type V

Filter:
  • Western Blot (WB) analysis of various lysates using COL5A3 Polyclonal antibody (30204-1-AP)

    WB analysis using 30204-1-AP (same clone as 30204-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30204-1-AP (COL5A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30204-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using COL5A3 Polyclonal antibody (30204-1-AP)

    IF Staining of HUVEC using 30204-1-AP (same clone as 30204-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using COL5A3 antibody (30204-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30204-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

30204-1-PBS bindet in WB, IF/ICC, Indirect ELISA COL5A3 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen COL5A3 fusion protein Ag30705
Vollständiger Name collagen, type V, alpha 3
Berechnetes Molekulargewicht 172 kDa
Beobachtetes Molekulargewicht145 kDa
GenBank-ZugangsnummerNM_015719
Gene symbol COL5A3
Gene ID (NCBI) 50509
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COL5A3 (Collagen type V alpha 3) belongs to the fibrillar collagen family. Type V collagen (COLV) is a member of a group I collagen (fibrillar forming collagen) and has three varieties of α chains: α1 (COL5A1), α2 (COL5A2), and α3 (COL5A3). COLV is a minor connective tissue component of nearly ubiquitous distribution. It binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. This antibody is specific to COL5A3.