COL6A1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

COL6A1 Polyklonal Antikörper für WB, IHC, IF, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(38)

Rezensionen (1)

Kat-Nr. : 17023-1-AP

Synonyme

COL6, collagen 6A1, Collagen alpha 1(VI) chain, Collagen alpha-1(VI) chain, Collagen Type VI

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  WB analysis of HEK-293T using 17023-1-AP

  WB result of Collagen Type VI antibody (17023-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Collagen Type VI transfected HEK-293T cells.

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  WB analysis using 17023-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17023-1-AP (Collagen Type VI antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 17023-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17023-1-AP (Collagen Type VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 17023-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 17023-1-AP (Collagen Type VI antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 17023-1-AP

  IP result of anti-Collagen Type VI (IP:17023-1-AP, 4ug; Detection:17023-1-AP 1:2000) with mouse heart tissue lysate 1050 ug.

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  IHC staining of human colon using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon using 17023-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Collagen Type VI antibody (17023-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon using 17023-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Collagen Type VI antibody (17023-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skeletal muscle using 17023-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Collagen Type VI antibody (17023-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse skeletal muscle tissue using 17023-1-AP

  IF result of anti-COL6A1 (17023-1-AP, 1:500) with PFA fixed mouse skeletal muscle tissue by Dr. Daniel Kopinke.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, HEK-293T-Zellen, humanes Herzgewebe, humanes Skelettmuskelgewebe, Rattenherzgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Herzgewebe, humanes Leberzirrhosegewebe, humanes Nierengewebe, humanes Lebergewebe, humanes Lungengewebe, humanes Eierstockgewebe, humanes Plazenta-Gewebe, humanes Hautkrebsgewebe, humanes Milzgewebe, humanes Hodengewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Kolongewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IF: Maus-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 23 publications below
• IHC: See 9 publications below
• IF: See 10 publications below

Produktinformation

17023-1-AP bindet in WB, IHC, IF, IF-P, IP, ELISA COL6A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COL6A1 fusion protein Ag10288
• Vollständiger Name: collagen, type VI, alpha 1
• Berechnetes Molekulargewicht: 1028 aa, 109 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC052575
• Gene symbol: COL6A1
• Gene ID (NCBI): 1291
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.

Publikationen

Species	Application	Title
mouse,rat	IF	Cell Death Differ; Developmental growth plate cartilage formation suppressed by artificial light at night via inhibiting BMAL1-driven collagen hydroxylation; Authors - Guangjin Chen
zebrafish	WB,IF	Cancer Res; Sarcoma Cells Secrete Hypoxia-Modified Collagen VI to Weaken the Lung Endothelial Barrier and Promote Metastasis; Authors - Ying Liu
human,mouse	WB,IF	Cell Rep; The mechano-chemical circuit in fibroblasts and dendritic cells drives basal cell proliferation in psoriasis; Authors - Jingwei Jiang
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
human	WB,IHC	Oncogene; Twist1-induced activation of human fibroblasts promotes matrix stiffness by upregulating palladin and collagen α1(VI).; Authors - I García-Palmero
human	WB,IHC	Cancer Gene Ther; Schwann cell derived pleiotrophin stimulates fibroblast for proliferation and excessive collagen deposition in plexiform neurofibroma; Authors - Zhuowei Tian

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Malak (Verified Customer) (02-24-2021)

It works well in the western blot

• Applications: Western Blot