COL6A3 Rekombinanter Antikörper

COL6A3 Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230540A11

Kat-Nr. : 83010-1-PBS

Synonyme

COL6A3-Specific, 230540A11, Collagen alpha 3(VI) chain, Collagen alpha-3(VI) chain, Collagen Type VI

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  WB analysis of A375 using 83010-1-RR (same clone as 83010-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 83010-1-RR (COL6A3-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83010-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83010-1-RR (same clone as 83010-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug COL6A3-Specific Recombinant antibody (83010-1-RR, Clone:230540A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83010-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83010-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83010-1-RR against Human COL6A3-Specific were performed. The affinity constant is 1.63 nM.

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Geprüfte Anwendungen

Produktinformation

83010-1-PBS bindet in WB, Cytometric bead array, Indirect ELISA COL6A3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: COL6A3 fusion protein Ag34752
• Vollständiger Name: collagen, type VI, alpha 3
• Berechnetes Molekulargewicht: 344 kDa
• Beobachtetes Molekulargewicht: 300 kDa
• GenBank-Zugangsnummer: NM_004369
• Gene symbol: COL6A3
• Gene ID (NCBI): 1293
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COL6A3 belongs to the type VI collagen family. Collagen VI acts as a cell-binding protein. It is expected to be expressed in smooth muscle. And the calculated molecular weight of COL6A3 is 343 kDa. The protein has similar expression in obese and T2DM (2 patients, and it regulates the chemotaxis and inflammation of macrophages in adipose tissue. Its expression is also related to weight gain. The expression of this protein in adipocytes is related to insulin resistance, which is believed to depend on PPAR (peroxisome proliferator-activated receptor) α-mediated adipocyte development (PMID: 25337653). Defects in COL6A3 are a cause of Bethlem myopathy (BM). Defects in COL6A3 are a cause of Ullrich congenital muscular dystrophy (UCMD) which also known as Ullrich scleroatonic muscular dystrophy. This antibody is specific to COL6A3.