COLEC11 Polyklonaler Antikörper

COLEC11 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15269-1-PBS

Synonyme

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  WB analysis of mouse liver using 15269-1-AP (same clone as 15269-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15269-1-AP (COLEC11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15269-1-PBS in a different storage buffer formulation.

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  WB analysis of human plasma using 15269-1-AP (same clone as 15269-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 15269-1-AP (COLEC11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15269-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 15269-1-AP (same clone as 15269-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15269-1-AP (COLEC11 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15269-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 15269-1-AP (same clone as 15269-1-PBS)

  IP result of anti-COLEC11 (IP:15269-1-AP, 4ug; Detection:15269-1-AP 1:500) with mouse liver tissue lysate 6400ug. This data was developed using the same antibody clone with 15269-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 15269-1-AP (same clone as 15269-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15269-1-AP (COLEC11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15269-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 15269-1-AP (same clone as 15269-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15269-1-AP (COLEC11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15269-1-PBS in a different storage buffer formulation.

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  IF Staining of ATCD5 cells using 15269-1-AP (same clone as 15269-1-PBS)

  IF result (confocal image) of CL-K1 in ATCD5 cell show Golgi localization from Prof. Philip L. Beales. This data was developed using the same antibody clone with 15269-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15269-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA COLEC11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COLEC11 fusion protein Ag7374
• Vollständiger Name: collectin sub-family member 11
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC000078
• Gene symbol: COLEC11
• Gene ID (NCBI): 78989
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COLEC11, also named as CL-K1 and Collectin-11, belongs to the COLEC10/COLEC11 family. It is a lectin that binds to various sugars: fucose > mannose. It does not bind to glucose, N-acetylglucosamine and N-acetylgalactosamine. COLEC11 binds to LPS. COLEC11 and MASP1 are two genes in the lectin complement pathway, they are mutated in 3MC syndrome, implicating this diverse inflammation-chemotaxis cascade in the etiology of human developmental disorders. COLEC11 serves as a guidance cue for neural crest cell migration.(PMID:21258343)