COMMD1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

COMMD1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(24)

Rezensionen (1)

Kat-Nr. : 11938-1-AP

Synonyme

Protein Murr1, MURR1, COMM domain-containing protein 1, C2orf5

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  WB analysis using 11938-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11938-1-AP (COMMD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11938-1-AP

  IP result of anti-COMMD1 (IP:11938-1-AP, 3ug; Detection:11938-1-AP 1:500) with mouse brain tissue lysate 5200ug.

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  IHC staining of human liver cancer using 11938-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11938-1-AP (COMMD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11938-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11938-1-AP (COMMD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11938-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COMMD1 antibody (11938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 20 publications below
• IHC: See 4 publications below
• IF: See 5 publications below
• CoIP: See 3 publications below

Produktinformation

11938-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA COMMD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COMMD1 fusion protein Ag2535
• Vollständiger Name: copper metabolism (Murr1) domain containing 1
• Berechnetes Molekulargewicht: 190 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC022046
• Gene symbol: COMMD1
• Gene ID (NCBI): 150684
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COMMD1(COMM domain-containing protein 1), also named MURR1, is implicated in copper homeostasis by binding one copper ion per monomer. COMMD1 was reported to accelerate the ubiquitination and degradation of NF-kappa-B subunits, its deficiency exerts enhanced NF-kappa-B mediated celluar response. Ubiquitinated by XIAP (X-linked inhibitor of apoptosis), COMMD1 undergoes proteasomal degradation. SOD1 activity can be downregulated by COMMD1.

Publikationen

Species	Application	Title
human,mouse	WB,IF	Circ Res; COMMD Family Regulates Plasma LDL Levels and Attenuates Atherosclerosis Through Stabilizing the CCC Complex in Endosomal LDLR Trafficking.; Authors - Alina Fedoseienko ; KD Validated
human	WB	Nat Commun; A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.; Authors - Yunkai Zhu
canine	WB,IF	Nat Commun; CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL.; Authors - Paulina Bartuzi ; KO Validated
human		J Cell Biol; HRS-WASH axis governs actin-mediated endosomal recycling and cell invasion.; Authors - Ewan MacDonald
human	WB,IF,CoIP	Cell Rep; COMMD5/HCaRG Hooks Endosomes on Cytoskeleton and Coordinates EGFR Trafficking.; Authors - Carole G Campion ; KD Validated
human	WB	Theranostics; SPTBN1 inhibits inflammatory responses and hepatocarcinogenesis via the stabilization of SOCS1 and downregulation of p65 in hepatocellular carcinoma.; Authors - Ling Lin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Emilia (Verified Customer) (11-30-2018)

works well for WB

• Applications: Western Blot,
• Primary Antibody Dilution: 1;1000
• Cell Tissue Type: 293, Cos1