COMMD1 Monoklonaler Antikörper

COMMD1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3B10E10

Kat-Nr. : 67016-1-PBS

Synonyme

C2orf5, COMMD1, MURR1, Protein Murr1

---

• 

  WB analysis using 67016-1-Ig (same clone as 67016-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67016-1-Ig (COMMD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67016-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67016-1-Ig (same clone as 67016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67016-1-Ig (COMMD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67016-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67016-1-Ig (same clone as 67016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67016-1-Ig (COMMD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67016-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67016-1-Ig (same clone as 67016-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COMMD1 antibody (67016-1-Ig, Clone: 3B10E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67016-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

67016-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA COMMD1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: COMMD1 fusion protein Ag28596
• Vollständiger Name: copper metabolism (Murr1) domain containing 1
• Berechnetes Molekulargewicht: 190 aa, 21 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC022046
• Gene symbol: COMMD1
• Gene ID (NCBI): 150684
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COMMD1(COMM domain-containing protein 1), also named MURR1, is implicated in copper homeostasis by binding one copper ion per monomer. COMMD1 was reported to accelerate the ubiquitination and degradation of NF-kappa-B subunits, its deficiency exerts enhanced NF-kappa-B mediated celluar response. Ubiquitinated by XIAP (X-linked inhibitor of apoptosis), COMMD1 undergoes proteasomal degradation. SOD1 activity can be downregulated by COMMD1.