COMMD5 Polyklonaler Antikörper

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COMMD5 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10393-1-PBS

Synonyme

COMM domain containing 5, COMM domain-containing protein 5, HCaRG, HT002, Hypertension-related calcium-regulated gene protein

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  WB analysis of mouse kidney using 10393-1-AP (same clone as 10393-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10393-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 10393-1-AP (same clone as 10393-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10393-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 10393-1-AP (same clone as 10393-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10393-1-PBS in a different storage buffer formulation.

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  WB analysis of human stomach using 10393-1-AP (same clone as 10393-1-PBS)

  human stomach tissue were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10393-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse stomach using 10393-1-AP (same clone as 10393-1-PBS)

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10393-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 10393-1-AP (same clone as 10393-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10393-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10393-1-AP (same clone as 10393-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10393-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 10393-1-AP (same clone as 10393-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10393-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse heart using 10393-1-AP (same clone as 10393-1-PBS)

  IP result of anti-COMMD5 (IP:10393-1-AP, 4ug; Detection:10393-1-AP 1:500) with mouse heart tissue lysate 3200ug. This data was developed using the same antibody clone with 10393-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10393-1-AP (same clone as 10393-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COMMD5 antibody (10393-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10393-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10393-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA COMMD5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COMMD5 fusion protein Ag0592
• Vollständiger Name: COMM domain containing 5
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC003055
• Gene symbol: COMMD5
• Gene ID (NCBI): 28991
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COMM domain containing 5 (COMMD5, synonyms: HCARG, HT002) is a hypertension-related calcium-regulated gene. COMMD5 is negatively regulated by extracellular calcium concentration, and its basal mRNA levels were higher in hypertensive animals. Tissue distribution of COMMD5 shows a preponderance in the heart, stomach, jejunum, kidney (tubular fraction), liver, and adrenal gland (mainly in the medulla). COMMD5 mRNA is significantly more expressed in adult than in fetal organs, and its levels are decreased in tumors and cancerous cell lines. COMMD5 protein has no transmembrane domain, but contains 67% -helix content, a calcium-binding site, four putative "leucine zipper" motifs, and a nuclear receptor-binding domain. At the subcellular level, COMMD5 shows a nuclear localization.

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