COMP Polyklonaler Antikörper

COMP Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 28369-1-AP

Synonyme

TSP5, Cartilage oligomeric matrix protein, PSACH, Thrombospondin-5

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  WB analysis using 28369-1-AP

  Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 28369-1-AP (COMP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 28369-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28369-1-AP (COMP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 28369-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28369-1-AP (COMP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 28369-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28369-1-AP (COMP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 28369-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COMP antibody (28369-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of HepG2 using 28369-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug COMP Polyclonal antibody (28369-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, Hausschwein-Knorpelgewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

28369-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA COMP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COMP fusion protein Ag28749
• Vollständiger Name: cartilage oligomeric matrix protein
• Berechnetes Molekulargewicht: 757 aa, 83 kDa
• Beobachtetes Molekulargewicht: 80-110 kDa
• GenBank-Zugangsnummer: BC033676
• Gene symbol: COMP
• Gene ID (NCBI): 1311
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COMP is a secreted pentameric glycoprotein built from modular units and belongs to the thrombospondin protein family. COMP is expressed by hypertrophic chondrocytes and osteoblasts during endochondral ossification, and its expression is increased during fracture healing. COMP is involved in the assembly of collagen fibrils and other structural matrix components. COMP interacts with a number of cartilage ECM proteins, including fibronectin, collagen I, II IX, XII, and XIV, matrilins, as well as proteoglycans such as aggrecan and others.

Publikationen

Species	Application	Title
mouse,human	WB	Int J Chron Obstruct Pulmon Dis; Identification of Hub Genes Associated with COPD Through Integrated Bioinformatics Analysis.; Authors - Lin Chen
human	WB	Reprod Sci; Effect of GLIS1 on the Lymph Node Metastasis of Cervical Squamous Carcinoma Based on Transcriptome Analysis; Authors - Jiazhen Huang
human	IHC	Front Mol Biosci; Serum proteomic profiling of precancerous gastric lesions and early gastric cancer reveals signatures associated with systemic inflammatory response and metaplastic differentiation; Authors - Yueqing Gong
mouse	IF	Eur J Pharmacol; Endonuclear Circ-calm4 Regulates Ferroptosis via a circR-Loop of the COMP Gene in Pulmonary Artery Smooth Muscle Cells; Authors - Aijing Liu