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COMP Monoklonaler Antikörper

COMP Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D7A6

Kat-Nr. : 66793-1-PBS

Synonyme

TSP5, 1D7A6, Cartilage oligomeric matrix protein, PSACH, Thrombospondin-5

Filter:
  • Western Blot (WB) analysis of pig cartilage tissue using COMP Monoclonal antibody (66793-1-Ig)

    WB analysis of pig cartilage using 66793-1-Ig (same clone as 66793-1-PBS)

    pig cartilage tissue were subjected to SDS PAGE followed by western blot with 66793-1-Ig (COMP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66793-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using COMP Monoclonal antibody (66793-1-Ig)

    IF Staining of HepG2 using 66793-1-Ig (same clone as 66793-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COMP antibody (66793-1-Ig, Clone: 1D7A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66793-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using COMP Monoclonal antibody (66793-1-Ig)

    IF Staining of U2OS using 66793-1-Ig (same clone as 66793-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using COMP antibody (66793-1-Ig, Clone: 1D7A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66793-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

66793-1-PBS bindet in WB, IF/ICC, Indirect ELISA COMP und zeigt Reaktivität mit Hausschwein, human

Getestete Reaktivität Hausschwein, human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen COMP fusion protein Ag4575
Vollständiger Name cartilage oligomeric matrix protein
Berechnetes Molekulargewicht 757 aa, 83 kDa
Beobachtetes Molekulargewicht 100-130 kDa
GenBank-ZugangsnummerBC033676
Gene symbol COMP
Gene ID (NCBI) 1311
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COMP is a secreted pentameric glycoprotein built from modular units and belongs to the thrombospondin protein family. COMP is expressed by hypertrophic chondrocytes and osteoblasts during endochondral ossification, and its expression is increased during fracture healing. COMP is involved in the assembly of collagen fibrils and other structural matrix components. COMP interacts with a number of cartilage ECM proteins, including fibronectin, collagen I, II IX, XII, and XIV, matrilins, as well as proteoglycans such as aggrecan and others. This antibody got 100-130 kDa maybe due to glycosylation.