COMT Monoklonaler Antikörper

COMT Monoklonal Antikörper für WB, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A1A4

Kat-Nr. : 68181-1-PBS

Synonyme

catechol O methyltransferase, COMT

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  WB analysis using 68181-1-Ig (same clone as 68181-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68181-1-Ig (COMT antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68181-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 68181-1-Ig (same clone as 68181-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using COMT antibody (68181-1-Ig, Clone: 1A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68181-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68181-1-PBS bindet in WB, IF-P, Indirect ELISA COMT und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: COMT fusion protein Ag6502
• Vollständiger Name: catechol-O-methyltransferase
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 28-30 kDa
• GenBank-Zugangsnummer: BC000419
• Gene symbol: COMT
• Gene ID (NCBI): 1312
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Catechol O-methyltransferase (COMT) is an important enzyme that catalyzes O-methylation and inactivation of metabolically active catechol-containing bioactive molecules and drugs. The mammalian COMT enzyme exists in soluble form (24-26 kDa) and membrane-bound form (28-30 kDa), which are encoded by a single gene utilizing different promoters.