COPA Rekombinanter Antikörper

COPA Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230361B1

Kat-Nr. : 83089-2-PBS

Synonyme

HEP COP, FLJ26320, Coatomer subunit alpha, Alpha COP, Alpha coat protein

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  WB analysis using 83089-2-RR (same clone as 83089-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83089-2-RR (COPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83089-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 83089-2-RR (same clone as 83089-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83089-2-RR (COPA antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83089-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83089-2-RR (same clone as 83089-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using COPA antibody (83089-2-RR, Clone: 230361B1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83089-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83089-2-RR (same clone as 83089-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug COPA Recombinant antibody (83089-2-RR, Clone:230361B1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83089-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

83089-2-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA COPA und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: COPA fusion protein Ag34207
• Vollständiger Name: coatomer protein complex, subunit alpha
• Berechnetes Molekulargewicht: 138 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: NM_004371
• Gene symbol: COPA
• Gene ID (NCBI): 1314
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COPA, also named HEP-COP and Alpha-COP, is coatomer subunit alpha. The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.