COPA Rekombinanter Antikörper

COPA Rekombinant Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230361G2

Kat-Nr. : CL488-83089

Synonyme

HEP COP, FLJ26320, Coatomer subunit alpha, Alpha COP, Alpha coat protein

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  IF Staining of A549 using CL488-83089

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 COPA antibody (CL488-83089, Clone: 230361G2 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-83089

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug Coralite® Plus 488 COPA Recombinant Antibody (CL488-83089, Clone:230361G2)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: A549-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-83089 bindet in IF/ICC, FC (Intra) COPA und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: COPA fusion protein Ag34207
• Vollständiger Name: coatomer protein complex, subunit alpha
• Berechnetes Molekulargewicht: 138 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: NM_004371
• Gene symbol: COPA
• Gene ID (NCBI): 1314
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COPA, also named HEP-COP and Alpha-COP, is coatomer subunit alpha. The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.